液氮冷凍培養菌種的過程
液態氮(dan)(dan)氣(qi)，氣(qi)化為氮(dan)(dan)氣(qi)，的冷(leng)凍效果(guo)，在醫療科研上有廣泛的應用。液氮(dan)(dan)冷(leng)凍培養(yang)菌(jun)種有很好的效果(guo)。其主要方法(fa)如下(xia)：

液(ye)氮(dan)冷凍培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)菌種(zhong)(zhong)的過程：欲用液(ye)態(tai)(tai)氮(dan)保(bao)(bao)存(cun)的繭種(zhong)(zhong)將其在(zai)適(shi)宜培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)基適(shi)溫培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)至靜(jing)IL期(qi)前(qian)期(qi)，生芽孢的菌應培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)個(ge)形(xing)成(cheng)成(cheng)熟的芽孢為止(zhi)：取(qu)(qu)(qu)試(shi)管或flnl生長好的培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)物剛無(wu)菌生理鹽水(shui)洗下細胞(bao)制(zhi)成(cheng)懸液(ye).寸(cun)入(ru)(ru)·九(jiu)閑鳳竹叫’使(shi)(shi)之細胞(bao)分散(san)均勻。再加(jia)等量的保(bao)(bao)護劑(甘油20%或10%DMSO)，再允分混㈠。懸液(ye)制(zhi)得后無(wu)菌地分裝入(ru)(ru)巳滅菌容量為2ml的安(an)瓿，每安(an)瓿瓶分裝0.5ml。然后進行凍結和(he)保(bao)(bao)存(cun)‘使(shi)(shi)菌種(zhong)(zhong)經(jing)超低溫保(bao)(bao)存(cun)后需使(shi)(shi)用時從液(ye)態(tai)(tai)氮(dan)氣冰箱取(qu)(qu)(qu)出使(shi)(shi)用前(qian)需經(jing)解(jie)凍及復蘇(su)培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)-解(jie)凍需采取(qu)(qu)(qu)快速加(jia)溫，將盛有凍結樣品(pin)的安(an)瓿取(qu)(qu)(qu)出后立即(ji)放置38。C恒溫水(shui)浴真-I，在(zai)具：1’搖動3~5min便可(ke)融化(hua)、復蘇(su)培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)即(ji)將解(jie)凍后的細胞(bao)接種(zhong)(zhong)于該細菌適(shi)宜的斜面培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)基上(shang)適(shi)溫培(pei)(pei)(pei)養(yang)(yang)(yang)(yang)(yang)即(ji)可(ke)。

液氮(dan)的(de)(de)冷凍(dong)作用將會被廣泛的(de)(de)應用在各個領域，所以一定(ding)要好好的(de)(de)利用它(ta)!